两个品种马骨髓基因表达谱分析

doi:10.11843/j.issn.0366-6964.2016.05.008

Abstract

Abstract:

To investigate the differential genes expression of bone marrow between different horse breeds，expression profiling libraries of Mongolian Horse-bone marrow and Thoroughbred-bone marrow were constructed.We obtained 7 219 956 and 7 116 170 useful reads by the Illumina Miseq sequencing，respectively.The most of reads in the 2 expression profiling libraries were maped on chromosome 11，chromosome 8 and chromosome 1，and the least of reads were maped on chromosome 29，chromosome 30 and chromosome 31.318 differentially expressed genes were found in 2 expression profiling libraries，including 21 immune-related genes.The Gene Ontology analysis showed that the differentially expressed genes were related to transport，cell-cell signaling，biological process and cytoplasm，etc.The Kyoto Encyclopedia of Genes and Genomes analysis indicated that nervous system was the most differentiated KEGG term.These results will provide data basis for further studies on the horses disease resistance and immune mechanism.

CLC Number:

S821.2

ZHAO Yi-ping，ZHANG Yu-hong，LI Bei，HUANG Jin-long，BAI Dong-yi，ZHAO Qi-nan，SHIRAIGO Wunierfu，YANG Li-hua，WU Jing，BAO Wuyundalai，DUGARJAVIIN Mang-lai. Analysis of Gene Expression Profile of Bone Marrow in Two Horse Breeds[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 922-930.

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References

［1］中国畜禽遗传资源委员会.中国畜禽遗传资源志-马驴驼志［M］.北京：中国农业出版社，2011.
Chinese Domestic Animal Genetic Resources Committee.Animal genetic resources in China-horse donkeys camels［M］.Beijing：China Agriculture Press，2011.(in Chinese)
［2］YU K，SHU G，YUAN F，et al.Fatty acid and transcriptome profiling of longissimus dorsi muscles between pig breeds differing in meat quality［J］.Int J Biol Sci，2013，9(1)：108-118.
［3］ZHAO S，HULSEGGE B，HARDERS F L，et al.Functional analysis of inter-individual transcriptome differential expression in pig longissimus muscle［J］.J Anim Breed Genet，2013，130(1)：72-78.
［4］EZEH P C，LAUER F T，MACKENZIE D，et al.Arsenite selectively inhibits mouse bone marrow lymphoid progenitor cell development in vivo and in vitro and suppresses humoral immunity in vivo［J］.PLoS One，2014，9(4)：e93920.
［5］PAN Q，SHAI O，LEE L J，et al.Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing［J］.Nat Genet，2008，40(12)：1413-1415.
［6］王兴春，杨致荣，王敏，等.高通量测序技术及其应用［J］.中国生物工程杂志，2012，32(1)：109-114.
WANG X C，YANG Z R，WANG M，et al.High-throughput sequencing technology and its application［J］.China Biotechnology，2012，32(1)：109-114.(in Chinese)
［7］PARK K D，PARK J，KO J，et al.Whole transcriptome analyses of six thoroughbred horses before and after exercise using RNA-Seq［J］.BMC Genomics，2012，13：473.
［8］MCGIVNEY B A，MCGETTIGAN P A，BROWNE J A，et al.Characterization of the equine skeletal muscle transcriptome identifies novel functional responses to exercise training［J］.BMC Genomics，2010，11：398.
［9］芒来.马在中国［M］.香港：香港文化出版社，2009.
MANG L.Horse in China［M］.Hongkong：Hongkong Culture Press，2009.(in Chinese)
［10］WADE C M，GIULOTTO E，SIGURDSSON S.Genome sequence，comparative analysis，and population genetics of the domestic horse［J］.Science，2009，326(5954)：865-867.
［11］杨虹，马月辉，李蓓，等.马基因组研究进展［J］.遗传，2010，32(3)：211-218.
YANG H，MA Y H，LI P，et al.Progress on horse genome project［J］.Hereditas，2010，32(3)：211-218.(in Chinese)
［12］刘武艺.两个品种鸡胸肌不同发育阶段全基因组表达谱的系统分析［D］.北京：中国农业大学，2010.
LIU W Y.Systematic analysis of the global gene expression profiles in breast muscle tissues of two chicken breeds at different developmental stages［D］.Beijing：China Agricultural University，2010.(in Chinese)
［13］LI X J，YANG H，LI G X，et al.Transcriptome profile analysis of porcine adipose tissue by high-throughput sequencing［J］.Anim Genet，2012，43(2)：144-152.
［14］辉钱.利用表达谱芯片技术研究中外猪种不同时期肌肉组织中的表达差异基因［D］.武汉：华中农业大学，2012.
HUI Q.Microarray analysis of skeletal muscle at four stages in yorkshire and meishan pigs［D］.Wuhan：Huazhong Agricultural University，2012.(in Chinese)
［15］张冬杰，刘娣，汪亮，等.民猪和大白猪背最长肌差异表达基因的筛选与注释［J］.畜牧兽医学报，2013，44(2)：181-187.
ZHANG D J，LIU D，WANG L，et al.Screen and annotation of different expression genes in Min pig and Yorkshire pig Longissimus dorsi muscle［J］.Acta Veterinaria et Zootechnica Sinica，2013，44(2)：181-187.(in Chinese)
［16］OSHLACK A，WAKEFIELD M J.Transcript length bias in RNA-seq data confounds systems biology［J］.Biol Direct，2009，4：14.

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Analysis of Gene Expression Profile of Bone Marrow in Two Horse Breeds

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